Katner Lab
Common Techniques in Katner Lab
Undergraduate students in the Katner Lab learn the following techniques:
- Mammalian cell culture (specifically glioblastoma and hepatocellular carcinoma)
- Evaluation of FDA-approved drugs and novel drugs for cytotoxicity, cell invasion, and cell migration
- Protein quantification from cell lysates
- Western blot and ELISA
- Enzyme activity assays
- CRISPR gene editing
- Clonal expansion to generate cell libraries
- Genomic analysis on CRISPR edits
Undergraduate Student Presentations
Emilie Greene, Hunter Burgess, Meaghan Keohane, Austen Bayne, et al. (2023) Glypican-3 Shedding in Human Hepatocellular Carcinoma Cells. American Association for Cancer Research Annual Meeting, Orlando, FL.
Morgan Rud, Austen Bayne, Emilie Greene, et al. (2023) The Influence of Glypicans on Glioblastoma Tumor Progression. American Association for Cancer Research Annual Meeting, Orlando, FL.
Rachel Van Keulen, Jordan Kimmet, Morgan Rud, Jamie Rogers, Austen Bayne, et al. (2022) Polynuclear platinum compounds targeting glypicans reduce tumor progression in glioblastoma. American Association for Cancer Research Annual Meeting, New Orleans, LA.
Meaghan Keohane, Emilie Greene, Hunter Burgess, Austen Bayne, Stevan Colakovic, et al. (2022) Understanding How Notum Promotes Glypican-3 Shedding in Hepatocellular Carcinoma. American Association for Cancer Research Annual Meeting, New Orleans, LA.
Morgan Pitcher, Tehut Zewdu, Diamond Yusuf, Trishala Karmacharya, et al. (2020) Formulation of CRISPR Edited Cells to Evaluate Glypican-Targeting in Glioblastoma. ASBMB program. Abstract: https://doi.org/10.1096/fasebj.2020.34.s1.05718
Morgan Pitcher, Tehut Zewdu, et al. (2019) Formulation of CRISPR edited cells to evaluate proteoglycan interactions. American Association for Cancer Research Annual Meeting, Atlanta, GA.
Tehut Zewdu, Morgan Pitcher, et al. (2019) Clonal expansion of newly synthesized glypican-3 knockouts in glioblastoma using CRISPR editing. American Association for Cancer Research Annual Meeting, Atlanta, GA.
Additionally, all posters were presented at the Undergraduate Research Seminar, Minnesota State University, Mankato.
